Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 25232-1-AP (same clone as 25232-1-PBS)
WB result of CCAR1 antibody (25232-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR1 transfected HEK-293 cells. This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
WB analysis using 25232-1-AP (same clone as 25232-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 25232-1-AP (CCAR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 25232-1-AP (same clone as 25232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 25232-1-AP (same clone as 25232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 25232-1-AP (same clone as 25232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 25232-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CCAR1 Polyclonal antibody, PBS Only

CCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25232-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CARP 1, CARP1, CARP-1, Death inducer with SAP domain, DIS

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25232-1-PBS targets CCAR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18970 Product name: Recombinant human CCAR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 217-343 aa of BC132725 Sequence: QPLLKTPPAVLQPIAPQTTFGVQTQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERERERRRSRERSPQRKRSRERS 相同性解析による交差性が予測される生物種
Full Name	cell division cycle and apoptosis regulator 1
Calculated molecular weight	1150 aa, 133 kDa
Observed molecular weight	150 kDa
GenBank accession number	BC132725
Gene Symbol	CCAR1
Gene ID (NCBI)	55749
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IX12
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 25232-1-AP (same clone as 25232-1-PBS)

WB analysis using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of human breast cancer using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of human breast cancer using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of rat colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of rat colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of rat kidney using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of rat kidney using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse kidney using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse kidney using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse liver using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse liver using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of mouse colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of human colon using 25232-1-AP (same clone as 25232-1-PBS)

IHC staining of human colon using 25232-1-AP (same clone as 25232-1-PBS)

IF Staining of A431 using 25232-1-AP (same clone as 25232-1-PBS)

IF Staining of A431 using 25232-1-AP (same clone as 25232-1-PBS)

CCAR1 Polyclonal antibody, PBS Only

CCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™