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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cell using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of HEK-293 cell using 18544-1-AP (same clone as 18544-1-PBS)

    WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE). This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of PC-3 using 18544-1-AP (same clone as 18544-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of HEK-293 using 18544-1-AP (same clone as 18544-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of PC-3 using 18544-1-AP (same clone as 18544-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX4 Polyclonal antibody (18544-1-AP)

    IF Staining of HeLa using 18544-1-AP (same clone as 18544-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX4 antibody (18544-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CBX4 Polyclonal antibody, PBS Only

CBX4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 18544-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18544-1-PBS targets CBX4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX4 fusion protein Ag13425 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 4 (Pc class homolog, Drosophila)
Calculated molecular weight 61 kDa
Observed molecular weight ~75 kDa
GenBank accession numberBC014967
Gene Symbol CBX4
Gene ID (NCBI) 8535
RRIDAB_2878552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00257
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.