Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31108-1-AP targets CBX1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34901 Product name: Recombinant human CBX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 60-115 aa of BC002609 Sequence: CPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFAR 相同性解析による交差性が予測される生物種 |
| Full Name | chromobox homolog 1 (HP1 beta homolog Drosophila ) |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 21-27 kDa |
| GenBank accession number | BC002609 |
| Gene Symbol | CBX1 |
| Gene ID (NCBI) | 10951 |
| RRID | AB_3669859 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P83916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CBX1 (chromobox 1), also known as CBX. It is expected to be located in nucleus, the protein is expressed in all adult and embryonic tissues. It is a member of the heterochromatin protein family. CBX1 is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. It may play an important role in the epigenetic control of chromatin structure and gene expression. The calculated molecular weight of CBX1 is 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBX1 antibody 31108-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
