Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10241-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10241-2-AP
WB result of CBX1 antibody (10241-2-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells.
WB analysis of A431 using 10241-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10241-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10241-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10241-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10241-2-AP
IP result of anti-CBX1 (IP:10241-2-AP, 3ug; Detection:10241-2-AP 1:500) with HEK-293 cells lysate 1480ug.
IHC staining of human stomach cancer using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 10241-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 10241-2-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CBX1 antibody (10241-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

Featured Product
KD/KO Validated

CBX1 Polyclonal antibody

CBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10241-2-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, HEK-293 cells, human brain tissue, NIH/3T3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human stomach cancer tissue, mouse testis tissue, human colon cancer tissue, human lung cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 2 publications below

Product Information

10241-2-AP targets CBX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0328 Product name: Recombinant human CBX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-185 aa of BC002609 Sequence: NKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDKKDDKN 相同性解析による交差性が予測される生物種
Full Name	chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated molecular weight	27 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC002609
Gene Symbol	CBX1
Gene ID (NCBI)	10951
RRID	AB_2228586
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P83916
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

Protocols

Product Specific Protocols
IF protocol for CBX1 antibody 10241-2-AP	Download protocol
IHC protocol for CBX1 antibody 10241-2-AP	Download protocol
IP protocol for CBX1 antibody 10241-2-AP	Download protocol
WB protocol for CBX1 antibody 10241-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP	J Exp Med ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons. Authors - Longfei Ma View Article
mouse	WB	Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Authors - Guang Yang View Article
human	WB,IHC,IF	J Cell Biol Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins. Authors - Jiang Wei-Qin WQ KD Validated View Article
human	WB	J Virol PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat. Authors - Zhangping He View Article
human	WB,IF	Front Genet Identification and Validation of Chromobox Family Members as Potential Prognostic Biomarkers and Therapeutic Targets for Human Esophageal Cancer. Authors - Xuefen Fang View Article
human	WB,IHC,IP	Adv Sci (Weinh) N⁶ -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation Authors - Yin Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10241-2-AP

WB analysis of MCF-7 using 10241-2-AP

WB analysis of A431 using 10241-2-AP

WB analysis of HEK-293 using 10241-2-AP

WB analysis of HEK-293 using 10241-2-AP

WB analysis of human brain using 10241-2-AP

IP experiment of HEK-293 using 10241-2-AP

IHC staining of human stomach cancer using 10241-2-AP

IHC staining of mouse testis using 10241-2-AP

IHC staining of mouse testis using 10241-2-AP

IHC staining of human colon cancer using 10241-2-AP

IHC staining of human colon cancer using 10241-2-AP

IHC staining of human lung cancer using 10241-2-AP

IHC staining of human lung cancer using 10241-2-AP

IHC staining of human ovary tumor using 10241-2-AP

IHC staining of human ovary tumor using 10241-2-AP

IHC staining of human stomach cancer using 10241-2-AP

IF Staining of HEK-293 using 10241-2-AP

CBX1 Polyclonal antibody

CBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.

PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.

Identification and Validation of Chromobox Family Members as Potential Prognostic Biomarkers and Therapeutic Targets for Human Esophageal Cancer.

N6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation

N⁶ -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation