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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis using 67861-1-Ig (same clone as 67861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of NCI-H1299 using 67861-1-Ig (same clone as 67861-1-PBS)

    WB result of CBS antibody (67861-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected NCI-H1299 cells. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of HEK-293 using 67861-1-Ig (same clone as 67861-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of Jurkat using 67861-1-Ig (same clone as 67861-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of K-562 using 67861-1-Ig (same clone as 67861-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CBS Monoclonal antibody (67861-1-Ig)

    IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CBS Monoclonal antibody (67861-1-Ig)

    IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBS Monoclonal antibody (67861-1-Ig)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBS Monoclonal antibody (67861-1-Ig)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CBS Monoclonal antibody, PBS Only

CBS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67861-1-PBS
Clone No.1E4F10

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:4.2.1.22, Cystathionine beta-synthase, cystathionine beta synthase, Beta-thionase, Beta thionase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67861-1-PBS targets CBS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7008

Product name: Recombinant human CBS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 206-551 aa of BC000440

Sequence: VAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK

相同性解析による交差性が予測される生物種
Full Name cystathionine-beta-synthase
Calculated molecular weight 61 kDa
Observed molecular weight 61-63 kDa
GenBank accession numberBC000440
Gene Symbol CBS
Gene ID (NCBI) 875
RRIDAB_2918619
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35520
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

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