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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBR1 Recombinant antibody (83736-4-RR)

    WB analysis using 83736-4-RR (same clone as 83736-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83736-4-RR (CBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83736-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBR1 Recombinant antibody (83736-4-RR)

    IF Staining of HeLa using 83736-4-RR (same clone as 83736-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBR1 antibody (83736-4-RR, Clone: 240707G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83736-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83736-4-RR

    Affinity and Kinetic Characterization of 83736-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83736-4-RR against Human CBR1 were performed. The affinity constant is 13.7 nM.

CBR1 Recombinant monoclonal antibody, PBS Only

CBR1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83736-4-PBS
Clone No.240707G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 240707G1, 20-beta-hydroxysteroid dehydrogenase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83736-4-PBS targets CBR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34830

Product name: Recombinant human CBR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-276 aa of BC002511

Sequence: MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQ

相同性解析による交差性が予測される生物種
Full Name carbonyl reductase 1
Calculated molecular weight 30 kDa
GenBank accession numberBC002511
Gene Symbol CBR1
Gene ID (NCBI) 873
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP16152
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.