Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, U-87 MG cells, Neuro-2a cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18207-1-AP targets CBLN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12835 Product name: Recombinant human CBLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-193 aa of BC093692 Sequence: RGQNETEPIVLEGKCLVVCDSNPTSDPTGTALGISVRSGSAKVAFSAIRSTNHEPSEMSNRTMIIYFDQVLVNIGNNFDSERSTFIAPRKGIYSFNFHVVKVYNRQTIQVSLMLNGWPVISAFAGDQDVTREAASNGVLIQMEKGDRAYLKLERGNLMGGWKYSTFSGFLVFPL 相同性解析による交差性が予測される生物種 |
| Full Name | cerebellin 1 precursor |
| Calculated molecular weight | 193 aa, 21 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC093692 |
| Gene Symbol | CBLN1 |
| Gene ID (NCBI) | 869 |
| RRID | AB_3669294 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23435 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CBLN1 (Cerebellin-1) is also named as Precerebellin. CBLN1 is a newly identified synaptic organizer belonging to the C1q family. Cbln1 was recently identified as the missing ligand for the orphan glutamate receptor δ2 (GluD2) (PMID: 21342763). In the cerebellum, Cbln1 is produced and secreted from granule cells and works as a strong synapse organizer between Purkinje cells and parallel fibers, the axons of the granule cells (PMID: 24607546). The glycosylation modification of CBLN1 resulted in a larger molecular weight.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBLN1 antibody 18207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |