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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBLL1 Polyclonal antibody (21179-1-AP)

    WB analysis using 21179-1-AP (same clone as 21179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21179-1-AP (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CBLL1 Polyclonal antibody (21179-1-AP)

    IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CBLL1 Polyclonal antibody (21179-1-AP)

    IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CBLL1 Polyclonal antibody (21179-1-AP)

    IHC staining of human lung cancer using 21179-1-AP (same clone as 21179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBLL1 Polyclonal antibody (21179-1-AP)

    IF Staining of HepG2 using 21179-1-AP (same clone as 21179-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody (21179-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CBLL1 Polyclonal antibody, PBS Only

CBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21179-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21179-1-PBS targets CBLL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15463

Product name: Recombinant human CBLL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC027460

Sequence: TDNELQGTNSSGSLGGLDVRRRIPIKLISKQANKAKPAPRTQRTINRMPAKAPPGDEEGFDYNEEERYDCKGGELFANQRRFPGHLFWDFQINILGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQCTRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEIPERFIMPPDKHHMS

相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC027460
Gene Symbol CBLL1
Gene ID (NCBI) 79872
RRIDAB_10732814
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ75N03
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

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