Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26890-1-AP targets CATSPER2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25283 Product name: Recombinant human CATSPER2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC064387 Sequence: MAAYQQEEQMQLPRADAIRSRLIDTFSLIEHLQGLSQAVPRHTIRELLDPSRQKKLVLGDQHQLVRFSIKPQRIEQISHAQRLLSRLHVRCSQRPPLSLWAGWVLECPLF 相同性解析による交差性が予測される生物種 |
| Full Name | cation channel, sperm associated 2 |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC064387 |
| Gene Symbol | CATSPER2 |
| Gene ID (NCBI) | 117155 |
| RRID | AB_3742203 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P56 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cation channel sperm-associated protein 2 (CATSPER2) is a pore-forming subunit of the CatSper complex, a sperm-specific voltage-gated calcium channel, that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte (PMID: 21412338; 21412339).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CATSPER2 antibody 26890-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |