Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12722-1-AP targets CATSPER1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3417 Product name: Recombinant human CATSPER1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-302 aa of BC032950 Sequence: MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQALSSHVHQSHHHSEARNHVRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYSGFHQQSDSHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHHQVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHGDHPHHTQHHYHQTHRHRDYHQH 相同性解析による交差性が予測される生物種 |
| Full Name | cation channel, sperm associated 1 |
| Calculated molecular weight | 780 aa, 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC032950 |
| Gene Symbol | CATSPER1 |
| Gene ID (NCBI) | 117144 |
| RRID | AB_3669148 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cation channel sperm-associated protein 1 (CATSPER1) belongs to a family of four sperm-specific tetrameric voltage-gated cation channels that are highly conserved in humans and mice (PMID: 12402840; 12932298). Definitive evidence that CatSper1 functions as a voltage-gated calcium channel to facilitate hyperactivated sperm motility came from whole-cell patch-clamp measurements of spermatozoa (PMID: 16467839). CATSPER1 channel currents were potentiated in response to intracellular alkalinization, increasing the concentration of intraflagellar calcium and thereby inducing hyperactivity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CATSPER1 antibody 12722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |