Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 21260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21260-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21260-1-AP
WB result of Catalase antibody (21260-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells.
WB analysis of Raji using 21260-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 21260-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 21260-1-AP (CAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21260-1-AP
IP result of anti-Catalase (IP:21260-1-AP, 4ug; Detection:21260-1-AP 1:10000) with HeLa cells lysate 1280 ug.
IHC staining of human liver cancer using 21260-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 21260-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 21260-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 21260-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 21260-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human hepatocirrhosis using 21260-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

Catalase Polyclonal antibody

Catalase Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21260-1-AP

Publications(259)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CAT, EC:1.11.1.6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (259)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, rat liver tissue, Raji cells, Jurkat cells, HepG2 cells, L02 cells, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 221 publications below
IHC	See 13 publications below
IF	See 19 publications below
FC	See 1 publications below

Product Information

21260-1-AP targets Catalase in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, chicken, bovine, goat, bat, fungus, meretrix meretrix
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15383 Product name: Recombinant human Catalase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 228-527 aa of BC112219 Sequence: EAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL 相同性解析による交差性が予測される生物種
Full Name	catalase
Calculated molecular weight	60 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC112219
Gene Symbol	Catalase
Gene ID (NCBI)	847
RRID	AB_10733099
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04040
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

Protocols

Product Specific Protocols
IHC protocol for Catalase antibody 21260-1-AP	Download protocol
IP protocol for Catalase antibody 21260-1-AP	Download protocol
WB protocol for Catalase antibody 21260-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB	Nanomicro Lett A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns Authors - Yonghua Tang View Article
mouse	WB	Cell Metab Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes. Authors - Calvin S Carter View Article
mouse	WB	Adv Mater Functionalized Nanozyme Microcapsules Targeting Deafness Prevention via Mitochondrial Homeostasis Remodeling Authors - Shengjie Ge View Article
mouse	WB,IHC	Nat Aging Bifidobacterium adolescentis regulates catalase activity and host metabolism and improves healthspan and lifespan in multiple species Authors - Shujie Chen View Article
mouse	IF	Autophagy FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation Authors - Wei Cai View Article
human	WB	Nat Commun A mass spectrometry-based strategy allows signature metabolite identification in tear fluid from people with diabetic cataracts. Authors - Ziheng Qi View Article

Validation Data Gallery

WB analysis using 21260-1-AP

WB analysis of Jurkat using 21260-1-AP

WB analysis of HeLa using 21260-1-AP

WB analysis of Raji using 21260-1-AP

WB analysis of rat liver using 21260-1-AP

IP experiment of HeLa using 21260-1-AP

IHC staining of human liver cancer using 21260-1-AP

IHC staining of human liver cancer using 21260-1-AP

IHC staining of human liver cancer using 21260-1-AP

IHC staining of human liver cancer using 21260-1-AP

IHC staining of human hepatocirrhosis using 21260-1-AP

IHC staining of human hepatocirrhosis using 21260-1-AP

Catalase Polyclonal antibody

Catalase Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Functionalized Nanozyme Microcapsules Targeting Deafness Prevention via Mitochondrial Homeostasis Remodeling

Bifidobacterium adolescentis regulates catalase activity and host metabolism and improves healthspan and lifespan in multiple species

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation

A mass spectrometry-based strategy allows signature metabolite identification in tear fluid from people with diabetic cataracts.

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