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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Calpastatin Recombinant monoclonal antibody (86664-3-RR)

    WB analysis of HeLa using 86664-3-RR

    WB result of Calpastatin antibody (86664-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpastatin transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CAST Recombinant monoclonal antibody (86664-3-RR)

    WB analysis using 86664-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86664-3-RR (CAST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using Calpastatin Recombinant monoclonal antibody (86664-3-RR)

    IHC staining of human heart using 86664-3-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 86664-3-RR (Calpastatin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using Calpastatin Recombinant monoclonal antibody (86664-3-RR)

    IHC staining of human heart using 86664-3-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 86664-3-RR (Calpastatin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calpastatin Recombinant monoclonal antibody (86664-3-RR)

    IF Staining of HeLa using 86664-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (86664-3-RR, Clone: 251652G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86664-3-RR

    Affinity and Kinetic Characterization of 86664-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86664-3-RR against Human Calpastatin were performed. The affinity constant is 6.39 nM.

  • KD/KO Validated

Calpastatin Recombinant monoclonal antibody

Calpastatin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86664-3-RR
Clone No.251652G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CAST, Calpain inhibitor, Sperm BS-17 component

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MDA-MB-231 cells, A549 cells, HaCaT cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86664-3-RR targets Calpastatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2897

Product name: Recombinant human CAST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC013579

Sequence: MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEK

相同性解析による交差性が予測される生物種
Full Name calpastatin
Calculated molecular weight 667 aa, 72 kDa
Observed molecular weight110-130 kDa
GenBank accession numberBC013579
Gene Symbol Calpastatin
Gene ID (NCBI) 831
RRIDAB_3745040
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calpastatin (CAST) is an endogenous protein that specifically inhibits the activity of calpains, a family of calcium-dependent proteases involved in numerous cellular processes. The CAST gene encodes calpastatin, which contains multiple calpain-inhibiting domains that bind to the active site of calpain, preventing it from performing its functions. Calpastatin is found in the cytosol and plays a key role in regulating intracellular proteolysis, with malfunctions in the calpain-calpastatin system linked to various diseases, including autoimmune disorders and neurodegenerative conditions.

Protocols

Product Specific Protocols
IF protocol for Calpastatin antibody 86664-3-RRDownload protocol
IHC protocol for Calpastatin antibody 86664-3-RRDownload protocol
WB protocol for Calpastatin antibody 86664-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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