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Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA
Conjugate
Unconjugated
2181
Cat no : 19677-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, mouse spleen tissue, HeLa cells, Staurosporine treated Jurkat cells, rat brain tissue, rat liver tissue |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue, mouse eye tissue |
Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 2013 publications below |
IHC | See 199 publications below |
IF | See 101 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
RIP | See 1 publications below |
Product Information
19677-1-AP targets Caspase 3/p17/p19 in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, monkey, chicken, zebrafish, hamster, sheep, goat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | caspase 3, apoptosis-related cysteine peptidase |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 32-35 kDa, 17 kDa, 19 kDa |
GenBank accession number | NM_004346 |
Gene symbol | Caspase 3 |
Gene ID (NCBI) | 836 |
RRID | AB_10733244 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.
Protocols
Product Specific Protocols | |
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WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection | ||
Nat Commun Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. | ||
Neuron UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Charlotte (Verified Customer) (08-15-2024) | Done in milk. Many bands but the most intense one matches the p32 of the caspase 3
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FH Alessandro (Verified Customer) (12-09-2023) | Reliable apoptosis Ab for IF
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FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.
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FH Hala (Verified Customer) (04-12-2021) | works very well
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FH Isha (Verified Customer) (02-03-2021) | Gave me crystal clear bands in kidney lysates. really happy with the product
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FH Diane (Verified Customer) (02-02-2021) | Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.
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FH Chao (Verified Customer) (03-12-2020) | Full-length but not cleaved isoform is detected by western blot
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FH Kishor (Verified Customer) (01-30-2019) | It is an excellent antibody, worked every time when I used and got satisfactory results.
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