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Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19677-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, mouse brain tissue, HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, goat, chicken, rat, sheep, mouse, monkey, rabbit, Zebrafish, Hamster|
|Host / Isotype||Rabbit / IgG|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32-35 kDa, 17 kDa, 19 kDa|
|GenBank accession number||NM_004346|
|Gene ID (NCBI)||836|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.
Hala (Verified Customer) (04-12-2021)
works very well
Isha (Verified Customer) (02-03-2021)
Gave me crystal clear bands in kidney lysates. really happy with the product
Diane (Verified Customer) (02-02-2021)
Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.
Chao (Verified Customer) (03-12-2020)
Full-length but not cleaved isoform is detected by western blot
Kishor (Verified Customer) (01-30-2019)
It is an excellent antibody, worked every time when I used and got satisfactory results.