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Caspase 3/p17/p19 Polyclonal antibody

Caspase 3/p17/p19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 19677-1-AP

Synonyms

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3


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Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, HeLa cells, Staurosporine treated Jurkat cells, rat brain tissue, rat liver tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue, mouse eye tissue
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19677-1-AP targets Caspase 3/p17/p19 in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, monkey, chicken, zebrafish, hamster, sheep, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 32 kDa
Observed molecular weight 32-35 kDa, 17 kDa, 19 kDa
GenBank accession numberNM_004346
Gene symbol Caspase 3
Gene ID (NCBI) 836
RRIDAB_10733244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.

Protocols

Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IF protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IP protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Cancer

hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Authors - Lei Xu
mouseWB,IHC

Bioact Mater

Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection

Authors - Yumei Que
mouse,ratWB

Nat Commun

Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury

Authors - Tianbao Ye
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanWB

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
WB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Charlotte (Verified Customer) (08-15-2024)

Done in milk. Many bands but the most intense one matches the p32 of the caspase 3

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: NIH-3T3
Caspase 3/p17/p19 Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:19677-1-AP)
FH

Alessandro (Verified Customer) (12-09-2023)

Reliable apoptosis Ab for IF

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: ROs
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NSC34
FH

Hala (Verified Customer) (04-12-2021)

works very well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hepg2 treated with ethoposide
FH

Isha (Verified Customer) (02-03-2021)

Gave me crystal clear bands in kidney lysates. really happy with the product

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Kidney
FH

Diane (Verified Customer) (02-02-2021)

Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Cell lysates - glioblastoma primary
Caspase 3/p17/p19 Antibody Western Blot validation (1:500 dilution) in Cell lysates - glioblastoma primary (Cat no:19677-1-AP)
FH

Chao (Verified Customer) (03-12-2020)

Full-length but not cleaved isoform is detected by western blot

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Kishor (Verified Customer) (01-30-2019)

It is an excellent antibody, worked every time when I used and got satisfactory results.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
Caspase 3/p17/p19 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:19677-1-AP)