Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, HepG2 cells, THP-1 cells, U-937 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | / HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 1177 publications below |
| IHC | See 180 publications below |
| IF | See 236 publications below |
| IP | See 3 publications below |
| FC | See 1 publications below |
Product Information
22915-1-AP targets Caspase 1/P20 in WB, IHC, IF/ICC, IP, ELISA, Cell treatment, Western Blot applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, rabbit, monkey, chicken, hamster, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11143 Product name: Recombinant human Caspase 1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-311 aa of BC062327 Sequence: MADKVLKEKRKLFIRSMGEAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH 相同性解析による交差性が予測される生物種 |
| Full Name | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
| Calculated molecular weight | 404 aa, 45 kDa |
| Observed molecular weight | 45-47 kDa, 30 kDa, 35 kDa |
| GenBank accession number | BC062327 |
| Gene Symbol | Caspase 1 |
| Gene ID (NCBI) | 834 |
| RRID | AB_2876874 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29466 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize p45 procaspase and the mature fragment p20.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Caspase 1/P20 antibody 22915-1-AP | Download protocol |
| WB protocol for Caspase 1/P20 antibody 22915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma. | ||
Signal Transduct Target Ther 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis | ||
Mol Cancer Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases | ||
Nat Chem Eng Hydrogen evolution and dynamics in hydrogel electrochemical cells for ischemia-reperfusion therapy. | ||
Adv Mater A Targeted, Tandem-Activated Artificial Urinary Biomarker Sensor for Early Detection and Therapeutic Intervention of Thoracic Aortic Aneurysm and Dissection. |