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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARHSP1 Recombinant monoclonal antibody (83300-3-RR)

    WB analysis using 83300-3-RR (same clone as 83300-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83300-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CARHSP1 Recombinant monoclonal antibody (83300-3-RR)

    WB analysis of HEK-293 using 83300-3-RR (same clone as 83300-3-PBS)

    WB result of CARHSP1 antibody (83300-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83300-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CARHSP1 Recombinant monoclonal antibody (83300-3-RR)

    FC experiment of HepG2 using 83300-3-RR (same clone as 83300-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83300-3-PBS in a different storage buffer formulation.

CARHSP1 Recombinant monoclonal antibody, PBS Only

CARHSP1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83300-3-PBS
Clone No.230489A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

230489A2, Calcium-regulated heat-stable protein 1, Calcium-regulated heat-stable protein of 24 kDa, CRHSP 24, CRHSP-24

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83300-3-PBS targets CARHSP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2265

Product name: Recombinant human CARHSP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-147 aa of BC003366

Sequence: MSSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

相同性解析による交差性が予測される生物種
Full Name calcium regulated heat stable protein 1, 24kDa
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight22 kDa
GenBank accession numberBC003366
Gene Symbol CARHSP1
Gene ID (NCBI) 23589
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2V2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).

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