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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using CARHSP1 Recombinant antibody (83300-2-RR)

    IHC staining of mouse testis using 83300-2-RR (same clone as 83300-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83300-2-RR (CARHSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CARHSP1 Recombinant antibody (83300-2-RR)

    IF Staining of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CARHSP1 antibody (83300-2-RR, Clone: 230489F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CARHSP1 Recombinant antibody (83300-2-RR)

    FC experiment of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-2-RR, Clone:230489F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83300-2-RR

    Affinity and Kinetic Characterization of 83300-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83300-2-RR against Human CARHSP1 were performed. The affinity constant is 4.36 nM.

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CARHSP1 Recombinant antibody, PBS Only

CARHSP1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83300-2-PBS
Clone No.230489F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83300-2-PBS targets CARHSP1 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CARHSP1 fusion protein Ag2265 相同性解析による交差性が予測される生物種
Full Name calcium regulated heat stable protein 1, 24kDa
Calculated molecular weight 147 aa, 16 kDa
GenBank accession numberBC003366
Gene Symbol CARHSP1
Gene ID (NCBI) 23589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2V2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.