Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse liver tissue, mouse testis tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11672-1-AP targets CARHSP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CARHSP1 fusion protein Ag2265 相同性解析による交差性が予測される生物種 |
| Full Name | calcium regulated heat stable protein 1, 24kDa |
| Calculated molecular weight | 147 aa, 16 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC003366 |
| Gene Symbol | CARHSP1 |
| Gene ID (NCBI) | 23589 |
| RRID | AB_3669130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2V2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CARHSP1 antibody 11672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |