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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARD9 Monoclonal antibody

CARD9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F3E10

Cat no : 68657-1-Ig

Synonyms

CARD9, hCARD9

Validation Data Gallery

Applications:
  • WB analysis using 68657-1-Ig

    WB analysis using 68657-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 68657-1-Ig

    WB analysis of U-937 using 68657-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of TF-1 using 68657-1-Ig

    WB analysis of TF-1 using 68657-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68657-1-Ig

    WB analysis using 68657-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNK-92 cells, HL-60 cells, TF-1 cells, U-937 cells, THP-1 cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68657-1-Ig targets CARD9 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CARD9 fusion protein Ag33643 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 9
Calculated molecular weight 62 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC008877
Gene symbol CARD9
Gene ID (NCBI) 64170
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

Protocols

Product Specific Protocols
WB protocol for CARD9 antibody 68657-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols