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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD8 Polyclonal antibody (14516-1-AP)

    WB analysis using 14516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14516-1-AP (CARD8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CARD8 Polyclonal antibody (14516-1-AP)

    IHC staining of human prostate cancer using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CARD8 Polyclonal antibody (14516-1-AP)

    IHC staining of human prostate cancer using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CARD8 Polyclonal antibody (14516-1-AP)

    IHC staining of human tonsillitis using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14516-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

DACAR, Caspase recruitment domain-containing protein 8, N-terminus, Caspase recruitment domain-containing protein 8, C-terminus, Caspase recruitment domain-containing protein 8, CARD-inhibitor of NF-kappa-B-activating ligand

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HUVEC cells, THP-1 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14516-1-AP targets CARD8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5975

Product name: Recombinant human CARD8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 10-341 aa of BC056891

Sequence: SSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLVDNSIRELQYTKTGIFFQAEACVTNDTVYRELPCVSETLCDISHFFQEDDETEAEPLLFRAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQIVSSYASKVCFEIEEDYKNRQFLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIAFGSWSQHLALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHSISLQAGEVDVSWFLVAHFKNEGMVLEHPARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHLYLVPSDA

相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 8
Calculated molecular weight61 kDa, 49 kDa
Observed molecular weight61 kDa, 49 kDa
GenBank accession numberBC056891
Gene Symbol CARD8
Gene ID (NCBI) 22900
RRIDAB_2068262
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2G2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CARD8 belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.

Protocols

Product Specific Protocols
IHC protocol for CARD8 antibody 14516-1-APDownload protocol
WB protocol for CARD8 antibody 14516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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