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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CARD6 Polyclonal antibody (18029-1-AP)

    WB analysis of HepG2 using 18029-1-AP (same clone as 18029-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18029-1-AP (CARD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CARD6 Polyclonal antibody (18029-1-AP)

    WB analysis of HepG2 using 18029-1-AP (same clone as 18029-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18029-1-AP (CARD6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human testis using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human testis using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human spleen using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human spleen using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human lung using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human lung using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human kidney using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CARD6 Polyclonal antibody (18029-1-AP)

    IHC staining of human kidney using 18029-1-AP (same clone as 18029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18029-1-PBS in a different storage buffer formulation.

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CARD6 Polyclonal antibody, PBS Only

CARD6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18029-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18029-1-PBS targets CARD6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD6 fusion protein Ag12595 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 6
Calculated molecular weight 1037 aa, 116 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC093825
Gene Symbol CARD6
Gene ID (NCBI) 84674
RRIDAB_2290686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX69
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The caspase recruitment domain (CARD) is a homotypic protein-protein interaction module that links components of signal transduction pathways implicated in the regulation of apoptosis or adaptive or innate immunity. Although much progress has been made in assigning precise roles to most CARD-containing proteins, the functions of the 1,037-amino-acid (aa) human and 1,175-aa mouse CARD6 proteins are still unknown. CARD6 has a unique structure in that it contains the CARD at the N terminus, a glutamic acid-rich region following the CARD, and a proline-rich region at the C terminus. CARD6 also harbors a 350-aa region with similarity to upregulated gene 4 (URG4), a protein that is induced in response to hepatitis Bx antigen overexpression and exerts a positive effect on proliferation. Both CARD6 and URG4 share structural features with members of the multifaceted, IFN-inducible GTPase (IFNiGTPase) superfamily, which contains some of the proteins most abundantly induced during cell-autonomous immune responses. The calcualted molecular weight of CARD is 116 kDa, but modified CARD6 is about 130 kDa. (PMID: 18160713)