Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
10400-1-AP targets CARD14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0628 Product name: Recombinant human CARD14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC018142 Sequence: MGELCRRDSALTALDEETLWEMMESHRHRIVRCICPSRLTPYLRQAKVLCQLDEEEVLHSPRLTNSAMRAGHLLDLLKTRGKNGAIAFLESLKFHNPDVYTLVTGLQPDVDFSNFSGLMETSKLTECLAGAIGSLQEELNQEKGQKEVLLRRCQQLQEHLGLAETRAEGLHQLEADHSRMKREVSAHFHEVLRLKDEMLSLSLHYSNALQEKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFSLAEKDILEQSLDEARGSRQE 相同性解析による交差性が予測される生物種 |
| Full Name | caspase recruitment domain family, member 14 |
| Calculated molecular weight | 113 kDa |
| Observed molecular weight | 113-140 kDa |
| GenBank accession number | BC018142 |
| Gene Symbol | CARD14 |
| Gene ID (NCBI) | 79092 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CARD14/CARMA2 is a novel caspase recruitment domain-containing protein 14 and is also a membrane-associated guanylate kinase (MAGUK) family member. CARD domain is a protein-binding element that mediates the assembly of CARD-containing proteins into apoptosis and NF-kappaB signaling complexes, which has been implicated in inflammatory disorders. CARD14 activates NF-kappaB signaling via interaction with BCL10. Defects in CARD14 are the cause of susceptibility to psoriasis type 2 (PSORS2), which is a common inflammatory disorder of the skin and other organs caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CARD14 antibody 10400-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene MALT1 is a critical mediator of PAR1-driven NF-κB activation and metastasis in multiple tumor types. | ||
Biomedicines CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells | ||
Biochem J CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation |