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- KD/KO Validated
CAPZB Polyclonal antibody
CAPZB Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 25043-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse testis tissue, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue |
Positive IF detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 5 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 25043-1-AP is CAPZB Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CAPZB fusion protein Ag19327 相同性解析による交差性が予測される生物種 |
Full Name | capping protein (actin filament) muscle Z-line, beta |
Calculated molecular weight | 277 aa, 31 kDa |
Observed molecular weight | 30-31 kDa |
GenBank accession number | BC109241 |
Gene symbol | CAPZB |
Gene ID (NCBI) | 832 |
RRID | AB_2879867 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CAPZB antibody 25043-1-AP | Download protocol |
IF protocol for CAPZB antibody 25043-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.
| ||
Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.
| ||
Development Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice. | ||
Cell Rep The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice. | ||
Cell Rep Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction. | ||
BMC Biol The temporal association of CapZ with early endosomes regulates endosomal trafficking and viral entry into host cells |