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Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using Calcyphosine Polyclonal antibody (16926-1-AP)

    WB analysis of U-251 using 16926-1-AP

    WB result of Calcyphosine antibody (16926-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calcyphosine transfected U-251 cells.

  • Western Blot (WB) analysis of various lysates using Calcyphosine Polyclonal antibody (16926-1-AP)

    WB analysis using 16926-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Calcyphosine Polyclonal antibody (16926-1-AP)

    WB analysis of human colon using 16926-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using Calcyphosine Polyclonal antibody (16926-1-AP)

    WB analysis of human spleen using 16926-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Calcyphosine Polyclonal antibody (16926-1-AP)

    IHC staining of human oesophagus cancer using 16926-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Calcyphosine Polyclonal antibody (16926-1-AP)

    IHC staining of human oesophagus cancer using 16926-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

Calcyphosine Polyclonal antibody

Calcyphosine Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16926-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CAPS, Calcyphosin, CAPS1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, human colon tissue, human spleen tissue, U-251 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16926-1-AP targets Calcyphosine in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10330

Product name: Recombinant human CAPS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-189 aa of BC080599

Sequence: MDAVDATMEKLRAQCLSRGASGIQGLARFFRQLDRDGSRSLDADEFRQGLAKLGLVLDQAEAEGVCRKWDRNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTVDDLRGVYSGRAHPKVRSGEWTEDEVLRRFLDNFDSSEKDGQVTLAEFQDYYSGVSASMNTDEEFVAMMTSAWQL

相同性解析による交差性が予測される生物種
Full Name calcyphosine
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC080599
Gene Symbol calcyphosine
Gene ID (NCBI) 828
RRIDAB_2070024
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.

Protocols

Product Specific Protocols
IHC protocol for Calcyphosine antibody 16926-1-APDownload protocol
WB protocol for Calcyphosine antibody 16926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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