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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of A375 using 67838-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of HeLa using 67838-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of K-562 using 67838-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of THP-1 using 67838-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of HSC-T6 using 67838-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of PC-12 using 67838-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    WB analysis of A431 using 67838-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CAPNS1 Monoclonal antibody (67838-1-Ig)

    IF Staining of A431 using 67838-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPNS1 antibody (67838-1-Ig, Clone: 2B6G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CAPNS1 Monoclonal antibody

CAPNS1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67838-1-Ig
Clone No.2B6G6

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, A431 cells, HeLa cells, K-562 cells, THP-1 cells, HSC-T6 cells, PC-12 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67838-1-Ig targets CAPNS1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21885

Product name: Recombinant human CAPNS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-323 aa of BC011903

Sequence: MFLVNSFLKGGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEVRTPILGYGCLGGPHPSALHTSSELQSPSSYFASRPWVRAKGLVLLGFPVLTLHPPLPSGCS

相同性解析による交差性が予測される生物種
Full Name calpain, small subunit 1
Calculated molecular weight 28 kDa
GenBank accession numberBC011903
Gene Symbol CAPNS1
Gene ID (NCBI) 826
RRIDAB_2918600
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP04632
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAPNS1 antibody 67838-1-IgDownload protocol
IF protocol for CAPNS1 antibody 67838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols