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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAPN3 Polyclonal antibody (28476-1-AP)

    WB analysis using 28476-1-AP (same clone as 28476-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28476-1-AP (CAPN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CAPN3 Polyclonal antibody (28476-1-AP)

    IHC staining of mouse skeletal muscle using 28476-1-AP (same clone as 28476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28476-1-AP (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28476-1-PBS targets CAPN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPN3 fusion protein Ag29454 相同性解析による交差性が予測される生物種
Full Name calpain 3, (p94)
Observed molecular weight 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank accession numberBC146672
Gene Symbol CAPN3
Gene ID (NCBI) 825
RRIDAB_2881150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20807
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).