A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

human placenta tissue were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.