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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis using 67412-1-Ig (same clone as 67412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of HepG2 using 67412-1-Ig (same clone as 67412-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of PC-3 using 67412-1-Ig (same clone as 67412-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of COLO 320 using 67412-1-Ig (same clone as 67412-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of A549 using 67412-1-Ig (same clone as 67412-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of HeLa using 67412-1-Ig (same clone as 67412-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

CAP2 Monoclonal antibody, PBS Only

CAP2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67412-1-PBS
Clone No.1G7D2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

1G7D2, Adenylyl cyclase associated protein 2, Adenylyl cyclase-associated protein 2, CAP 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67412-1-PBS targets CAP2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8622

Product name: Recombinant human CAP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 129-477 aa of BC008481

Sequence: MFNHLSAVSESIPALGWIAVSPKPGPYVKEMNDAATFYTNRVLKDYKHSDLRHVDWVKSYLNIWSELQAYIKEHHTTGLTWSKTGPVASTVSAFSVLSSGPGLPPPPPPLPPPGPPPLFENEGKKEESSPSRSALFAQLNQGEAITKGLRHVTDDQKTYKNPSLRAQGGQTQSPTKSHTPSPTSPKSYPSQKHAPVLELEGKKWRVEYQEDRNDLVISETELKQVAYIFKCEKSTIQIKGKVNSIIIDNCKKLGLVFDNVVGIVEVINSQDIQIQVMGRVPTISINKTEGCHIYLSEDALDCEIVSAKSSEMNILIPQDGDYREFPIPEQFKTAWDGSKLITEPAEIMA

相同性解析による交差性が予測される生物種
Full Name CAP, adenylate cyclase-associated protein, 2 (yeast)
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 51-53 kDa
GenBank accession numberBC008481
Gene Symbol CAP2
Gene ID (NCBI) 10486
RRIDAB_2882653
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP40123
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.