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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    WB result of CAP1 antibody (68207-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig lung tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    IHC staining of mouse lung using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAP1 Monoclonal antibody (68207-1-Ig)

    IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAP1 Monoclonal antibody (68207-1-Ig)

    IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAP1 Monoclonal antibody (68207-1-Ig)

    IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CAP1 Monoclonal antibody, PBS Only

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68207-1-PBS
Clone No.2H3A10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68207-1-PBS targets CAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8876

Product name: Recombinant human CAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-475 aa of BC013963

Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

相同性解析による交差性が予測される生物種
Full Name CAP, adenylate cyclase-associated protein 1 (yeast)
Calculated molecular weight 475 aa, 52 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC013963
Gene Symbol CAP1
Gene ID (NCBI) 10487
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ01518
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

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