Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32282-1-AP targets CAND1 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35297 Product name: Recombinant human CAND1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 396-586 aa of NM_018448 Sequence: HAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVK 相同性解析による交差性が予測される生物種 |
| Full Name | cullin-associated and neddylation-dissociated 1 |
| Calculated molecular weight | 136 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | NM_018448 |
| Gene Symbol | CAND1 |
| Gene ID (NCBI) | 55832 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86VP6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CAND1 (Cullin-Associated and Neddylation-Dissociated 1) is a key regulatory protein involved in the proper functioning of the CRL (Cullin-RING ubiquitin ligase) family, which plays a crucial role in ubiquitin-mediated protein degradation (PMID: 18444905).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CAND1 antibody 32282-1-AP | Download protocol |
| WB protocol for CAND1 antibody 32282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |