×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using CAMKK1 Polyclonal antibody (13624-1-AP)

    WB analysis of Jurkat using 13624-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CAMKK1 Polyclonal antibody (13624-1-AP)

    IP experiment of Jurkat using 13624-1-AP

    IP result of anti-CAMKK1 (IP:13624-1-AP, 4ug; Detection:13624-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human placenta using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human rectal cancer using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of mouse heart using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of mouse lung using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of rat brain using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of rat heart using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of rat lung using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human rectal cancer using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of rat brain using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CAMKK1 Polyclonal antibody

CAMKK1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13624-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase kinase 1, Calcium/calmodulin-dependent protein kinase kinase alpha, CaM kinase IV kinase, CaM kinase kinase 1, CaM kinase kinase alpha

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue, mouse lung tissue, human rectal cancer tissue, rat brain tissue, human placenta tissue, mouse heart tissue, rat heart tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4534

Product name: Recombinant human CAMKK1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-348 aa of BC031647

Sequence: MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSST

相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase kinase 1, alpha
Calculated molecular weight 520 aa, 57 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC031647
Gene Symbol CAMKK1
Gene ID (NCBI) 84254
RRIDAB_2068403
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5S9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcium/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda and involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory. This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 .

Protocols

Product Specific Protocols
IHC protocol for CAMKK1 antibody 13624-1-APDownload protocol
IP protocol for CAMKK1 antibody 13624-1-APDownload protocol
WB protocol for CAMKK1 antibody 13624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...