Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Hela cells, mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | human skeletal muscle tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55218-1-AP targets CAMK2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | calcium/calmodulin-dependent protein kinase II beta |
| Calculated molecular weight | 73 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | NM_001220 |
| Gene Symbol | CaMKII beta |
| Gene ID (NCBI) | 816 |
| RRID | AB_11183766 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CaMKII beta (Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAMK2B, CAM2 or CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals (PMID:22578382). CAMK2B has 8 isoforms produced by alternative splicing with the molecular weight 50-73 kDa. This antibody is specific to CAMK2B and can recognize all the isoforms except isofom 7.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAMK2B antibody 55218-1-AP | Download protocol |
| IHC protocol for CAMK2B antibody 55218-1-AP | Download protocol |
| WB protocol for CAMK2B antibody 55218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
