Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of pig cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of rat cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of mouse cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66843-1-Ig (same clone as 66843-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66843-1-Ig (same clone as 66843-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

CaMKII alpha Monoclonal antibody, PBS Only

CaMKII alpha Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66843-1-PBS

Clone No.1E3B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMK-II subunit alpha

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66843-1-PBS targets CaMKII alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag4736 Product name: Recombinant human CAMK2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-271 aa of BC040457 Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI 相同性解析による交差性が予測される生物種
Full Name	calcium/calmodulin-dependent protein kinase II alpha
Calculated molecular weight	478 aa, 54 kDa
Observed molecular weight	45-60 kDa
GenBank accession number	BC040457
Gene Symbol	CaMKII alpha
Gene ID (NCBI)	815
RRID	AB_2882184
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9UQM7
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa. This antibody can recognize all the members of the CAMK2 family.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis of pig cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis of rat cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis of mouse cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis of pig brain using 66843-1-Ig (same clone as 66843-1-PBS)

WB analysis of rat brain using 66843-1-Ig (same clone as 66843-1-PBS)

IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)