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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis of HeLa using 13613-1-AP

    WB result of CAMK1D antibody (13613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMK1D transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis using 13613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis of mouse pancreas using 13613-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis of mouse thymus using 13613-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of human pancreas using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of human pancreas using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CAMK1D Polyclonal antibody

CAMK1D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13613-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase type 1D, CaM K1, CaM KI delta, CaM kinase I delta, CaM kinase ID

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, HeLa cells, mouse thymus tissue, rat pancreas tissue
Positive IHC detected inmouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13613-1-AP targets CAMK1D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4517

Product name: Recombinant human CAMK1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4-198 aa of BC035745

Sequence: ENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPY

相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase ID
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC035745
Gene Symbol CAMK1D
Gene ID (NCBI) 57118
RRIDAB_2228498
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IU85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAMK1D (calcium/calmodulin-dependent protein kinase ID) is a serine/threonine kinase belonging to the calcium/calmodulin-dependent protein kinase 1 family. It functions as a component of a calcium-triggered signaling cascade, playing roles in regulating granulocyte function, activating CREB-dependent gene transcription, and influencing neutrophil chemotaxis and apoptosis. CAMK1D is involved in diverse biological processes including nervous system development and inflammatory responses.

Protocols

Product Specific Protocols
IHC protocol for CAMK1D antibody 13613-1-APDownload protocol
WB protocol for CAMK1D antibody 13613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Podocyte-specific KLF6 primes proximal tubule CaMK1D signaling to attenuate diabetic kidney disease

Authors - Nehaben A Gujarati
View Article
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