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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using CALML6 Polyclonal antibody (22274-1-AP)

    WB analysis of human heart using 22274-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 22274-1-AP (CALML6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using CALML6 Polyclonal antibody (22274-1-AP)

    WB analysis of pig heart using 22274-1-AP

    pig heart tissue were subjected to SDS PAGE followed by western blot with 22274-1-AP (CALML6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using CALML6 Polyclonal antibody (22274-1-AP)

    IHC staining of human heart using 22274-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22274-1-AP (CALML6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CALML6 Polyclonal antibody (22274-1-AP)

    IHC staining of human heart using 22274-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22274-1-AP (CALML6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using CALML6 Polyclonal antibody (22274-1-AP)

    IHC staining of human prostate hyperplasia using 22274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22274-1-AP (CALML6 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using CALML6 Polyclonal antibody (22274-1-AP)

    IHC staining of human prostate hyperplasia using 22274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22274-1-AP (CALML6 antibody) at dilution of 1:200 (under 40x lens).

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CALML6 Polyclonal antibody

CALML6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22274-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Synonyms

Calmodulin like protein 6, calmodulin like 6, CALGP, Calglandulin-like protein, Calglandulin like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue, pig heart tissue
Positive IHC detected inhuman heart tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

22274-1-AP targets CALML6 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name calmodulin-like 6
Calculated molecular weight 21 kDa
Observed molecular weight21 kDa
GenBank accession numberNM_138705
Gene Symbol CALML6
Gene ID (NCBI) 163688
RRIDAB_11182942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALML6, also named as CAGLP, CALGP , belongs to the calmodulin family and Calglandulin subfamily. It contains 4 EF-hand domains. The antibody is specific to CALML6.

Protocols

Product Specific Protocols
WB protocol for CALML6 antibody 22274-1-APDownload protocol
IHC protocol for CALML6 antibody 22274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Technol Cancer Res Treat

A Pyroptosis-Related Gene Signature Predicts Prognosis and Tumor Immune Microenvironment in Colorectal Cancer

Authors - Linjing Li
View Article
humanIHC

Gene

CALML6 as a potential diagnostic marker for thyroid cancer promotes thyroid cancer cell proliferation via modulating the immune microenvironment

Authors - Caixia Cheng
View Article