Validation Data Gallery
Tested Applications
| Positive IHC detected in | human skin cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
13059-1-AP targets CALML5 in IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3734 Product name: Recombinant human CALML5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-146 aa of BC039172 Sequence: MAGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDGDGDGEISFQEFLTAARKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFARMLAQE 相同性解析による交差性が予測される生物種 |
| Full Name | calmodulin-like 5 |
| Calculated molecular weight | 146 aa, 16 kDa |
| GenBank accession number | BC039172 |
| Gene Symbol | CALML5 |
| Gene ID (NCBI) | 51806 |
| RRID | AB_2069463 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CALML5, also known as calmodulin-like skin protein (CLSP), a recently discovered skin-specific calcium-binding protein, is closely related to keratinocyte differentiation. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CALML5 antibody 13059-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ann Diagn Pathol Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia. | ||
Elife Dalpiciclib partially abrogates ER signaling activation induced by pyrotinib in HER2+HR+ breast cancer |
