Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, PC-12 cells, mouse brain tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
20868-1-AP targets CALCR in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14920 Product name: Recombinant human CALCR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-164 aa of BC075028 Sequence: AFSNQTYPTIEPKPFLYVVGRKKMMDAQYKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYCDEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLV 相同性解析による交差性が予測される生物種 |
| Full Name | calcitonin receptor |
| Calculated molecular weight | 490 aa, 57 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC075028 |
| Gene Symbol | CALCR |
| Gene ID (NCBI) | 799 |
| RRID | AB_2878754 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30988 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The CALCR protein, also known as the calcitonin receptor, is a high-affinity receptor for the peptide hormone calcitonin and belongs to the subfamily of seven transmembrane-spanning G protein-coupled receptors. This protein plays a critical role in maintaining calcium homeostasis and regulating osteoclast-mediated bone resorption. The calcitonin receptor is involved in several physiological processes, including inhibition of osteoclastic bone resorption and increased renal calcium excretion, which helps to regulate Ca2+ concentrations. In addition, the CALCR protein has been detected in a variety of tissues not directly involved in the regulation of calcium homeostasis, with particular expression observed in the brain, suggesting distinct functions in the central nervous system (CNS) (PMID: 12730726).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CALCR antibody 20868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Cytisine attenuates bone loss of ovariectomy mouse by preventing RANKL-induced osteoclastogenesis. | ||
Mol Med A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone. | ||
Curr Issues Mol Biol COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer | ||
Adv Sci (Weinh) Enhanced Protein Damage Clearance Induces Broad Drug Resistance in Multitype of Cancers Revealed by an Evolution Drug-Resistant Model and Genome-Wide siRNA Screening. | ||
Nat Commun Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics | ||
Cell Metab Gut microbial alterations in arginine metabolism determine bone mechanical adaptation |