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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CALCOCO1 Recombinant antibody (84009-5-RR)

    IF Staining of A549 using 84009-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CALCOCO1 antibody (84009-5-RR, Clone: 241127B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HT-1080 cells using CALCOCO1 Recombinant antibody (84009-5-RR)

    IF Staining of HT-1080 using 84009-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using CALCOCO1 antibody (84009-5-RR, Clone: 241127B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84009-5-RR

    Affinity and Kinetic Characterization of 84009-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84009-5-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

CALCOCO1 Recombinant monoclonal antibody

CALCOCO1 Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 84009-5-RR
Clone No.241127B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

241127B3, Calcium-binding and coiled-coil domain-containing protein 1, Calphoglin, Cocoa, Coiled-coil coactivator protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHT-1080 cells, A549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84009-5-RR targets CALCOCO1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13876

Product name: Recombinant human CALCOCO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-691 aa of BC003177

Sequence: LKEQLRGAQELAASSQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVEAEKDKILKLSAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLPPYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAEDEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPATPTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFE

相同性解析による交差性が予測される生物種
Full Name calcium binding and coiled-coil domain 1
Calculated molecular weight 691 aa, 77 kDa
GenBank accession numberBC003177
Gene Symbol CALCOCO1
Gene ID (NCBI) 57658
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9P1Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CALCOCO1 antibody 84009-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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