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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALCOCO1 Recombinant antibody (84009-4-RR)

    WB analysis using 84009-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of rat testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of rat testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of mouse testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of mouse testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84009-4-RR

    Affinity and Kinetic Characterization of 84009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84009-4-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

CALCOCO1 Recombinant monoclonal antibody

CALCOCO1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84009-4-RR
Clone No.241127G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, MCF-7 cells, HeLa cells, HEK-293T cells, Ramos cells, rat brain tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84009-4-RR targets CALCOCO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13876

Product name: Recombinant human CALCOCO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-691 aa of BC003177

Sequence: LKEQLRGAQELAASSQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVEAEKDKILKLSAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLPPYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAEDEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPATPTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFE

相同性解析による交差性が予測される生物種
Full Name calcium binding and coiled-coil domain 1
Calculated molecular weight 691 aa, 77 kDa
Observed molecular weight100 kDa
GenBank accession numberBC003177
Gene Symbol CALCOCO1
Gene ID (NCBI) 57658
RRIDAB_3671577
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9P1Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
WB protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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