Validation Data Gallery
Tested Applications
| Positive WB detected in | TT cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
68774-1-Ig targets CALCA/Calcitonin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26141 Product name: Recombinant human CALCA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-116 aa of BC093753 Sequence: APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAP 相同性解析による交差性が予測される生物種 |
| Full Name | Calcitonin |
| Calculated molecular weight | 141 aa, 15 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC093753 |
| Gene Symbol | CALCA |
| Gene ID (NCBI) | 796 |
| RRID | AB_3670424 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01258 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CALCA/Calcitonin antibody 68774-1-Ig | Download protocol |
| WB protocol for CALCA/Calcitonin antibody 68774-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |