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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALCA/CGRP Polyclonal antibody (27257-1-AP)

    WB analysis using 27257-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 27257-1-AP (CALCA/CGRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of TT cells using CALCA/CGRP Polyclonal antibody (27257-1-AP)

    IP experiment of TT using 27257-1-AP

    IP result of anti-CALCA/CGRP (IP:27257-1-AP, 4ug; Detection:27257-1-AP 1:600) with TT cells lysate 1080 ug.

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CALCA/Calcitonin Polyclonal antibody

CALCA/Calcitonin Polyclonal Antibody for WB, IP, ELISA
Cat No. 27257-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CALCA, Calcitonin, CALC1, Calcitonin gene-related peptide 1, Calcitonin gene-related peptide I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTT cells
Positive IP detected inTT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27257-1-AP targets CALCA/Calcitonin in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCA/Calcitonin fusion protein Ag26133 相同性解析による交差性が予測される生物種
Full Name Calcitonin
Calculated molecular weight 141 aa, 15 kDa
Observed molecular weight14 kDa
GenBank accession numberBC093753
Gene Symbol CALCA
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.

Protocols

Product Specific Protocols
WB protocol for CALCA/Calcitonin antibody 27257-1-APDownload protocol
IP protocol for CALCA/Calcitonin antibody 27257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols