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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using CADM4 Polyclonal antibody (23136-1-AP)

    WB analysis of RAW264.7 using 23136-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23136-1-AP (CADM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CADM4 Polyclonal antibody (23136-1-AP)

    WB analysis of COLO 320 using 23136-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 23136-1-AP (CADM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CADM4 Polyclonal antibody (23136-1-AP)

    WB analysis of mouse lung using 23136-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 23136-1-AP (CADM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CADM4 Polyclonal antibody

CADM4 Polyclonal Antibody for WB, ELISA
Cat No. 23136-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CADM4, cell adhesion molecule 4, IGSF4C, Necl 4, NECL4, Nectin like protein 4, synCAM4, TSLC1 like protein 2, TSLL2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, RAW264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23136-1-AP targets CADM4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18922

Product name: Recombinant human CADM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-321 aa of BC141627

Sequence: AGQEVQTENVTVAEGGVAEITCRLHQYDGSIVVIQNPARQTLFFNGTRALKDERFQLEEFSPRRVRIRLSDARLEDEGGYFCQLYTEDTHHQIATLTVLVAPENPVVEVREQAVEGGEVELSCLVPRSRPAATLRWYRDRKELKGVSSSQENGKVWSVASTVRFRVDRKDDGGIIICEAQNQALPSGHSKQTQYVLDVQYSPTARIHASQAVVREGDTLVLTCAVTGNPRPNQIRWNRGNESLPERAEAVGETLTLPGLVSADNGTYTCEASNKHGHARALYVLVVYDPGAVVEAQTSV

相同性解析による交差性が予測される生物種
Full Name cell adhesion molecule 4
Calculated molecular weight 388 aa, 43 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC141627
Gene Symbol CADM4
Gene ID (NCBI) 199731
RRIDAB_2879215
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CADM4, also known as IGSF4C or TSLL2, is a cell adhesion molecule belonging to the immunoglobulin superfamily. CADM4 has been shown to serve as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for CADM4 antibody 23136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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