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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis using 15660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis of Jurkat using 15660-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis of SH-SY5Y using 15660-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CADM3 Polyclonal antibody

CADM3 Polyclonal Antibody for WB, ELISA
Cat No. 15660-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BIgR, Brain immunoglobulin receptor, CADM3, cell adhesion molecule 3, IGSF4B, Necl 1, NECL1, Nectin like protein 1, synCAM3, TSLC1 like protein 1, TSLL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15660-1-AP targets CADM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8081

Product name: Recombinant human CADM3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-398 aa of BC033819

Sequence: MGAPAASLLLLLLLFACCWAPGGANLSQDDSQPWTSDETVVAGGTVVLKCQVKDHEDSSLQWSNPAQQTLYFGEKRALRDNRIQLVTSTPHELSISISNVALADEGEYTCSIFTMPVRTAKSLVTVLGIPQKPIITGYKSSLREKDTATLNCQSSGSKPAARLTWRKGDQELHGEPTRIQEDPNGKTFTVSSSVTFQVTREDDGASIVCSVNHESLKGADRSTSQRIEVLYTPTAMIRPDPPHPREGQKLLLHCEGRGNPVPQQYLWEKEGSVPPLKMTQESALIFPFLNKSDSGTYGCTATSNMGSYKAYYTLNVNDPSPVPSSSSTYHAIIGGIVAFIVFLLLIMLIFLGHYLIRHKGTYLTHEAKGSDDAPDADTAIINAEGGQSGGDDKKEYFI

相同性解析による交差性が予測される生物種
Full Name cell adhesion molecule 3
Calculated molecular weight 43 kDa
Observed molecular weight 38-50 kDa
GenBank accession numberBC033819
Gene Symbol CADM3
Gene ID (NCBI) 57863
RRIDAB_2068044
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CADM3, also known as IGSF4B or TSLL1, is a transmembrane protein that is involved in the cell-cell adhesion. CADM3 is mainly expressed in the nervous system and plays a role in the formation of synapses, axon bundles, myelinated axons and cerebellar morphogenesis (PMID: 18420026).

Protocols

Product Specific Protocols
WB protocol for CADM3 antibody 15660-1-APDownload protocol
FC protocol for CADM3 antibody 15660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols