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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CADM1 Polyclonal antibody (14335-1-AP)

    WB analysis of mouse liver using 14335-1-AP (same clone as 14335-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CADM1 Polyclonal antibody (14335-1-AP)

    WB analysis of human placenta using 14335-1-AP (same clone as 14335-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CADM1 Polyclonal antibody (14335-1-AP)

    WB analysis of A549 using 14335-1-AP (same clone as 14335-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using CADM1 Polyclonal antibody (14335-1-AP)

    WB analysis of mouse lung using 14335-1-AP (same clone as 14335-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CADM1 Polyclonal antibody (14335-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14335-1-AP (same clone as 14335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14335-1-AP (CADM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CADM1 Polyclonal antibody (14335-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14335-1-AP (same clone as 14335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14335-1-AP (CADM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

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CADM1 Polyclonal antibody, PBS Only

CADM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14335-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NECL-2, NECL2, Necl 2, IGSF4A, IgSF4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14335-1-PBS targets CADM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CADM1 fusion protein Ag5621 相同性解析による交差性が予測される生物種
Full Name cell adhesion molecule 1
Calculated molecular weight 49 kDa
Observed molecular weight 49-60 kDa
GenBank accession numberBC047021
Gene Symbol CADM1
Gene ID (NCBI) 23705
RRIDAB_2243801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY67
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CADM1 (cell adhesion molecule 1), also referred to as SgIGSF, Necl-2 or TSLC-1, is a cell adhesion molecule belonging to the immunoglobulin superfamily. It can mediate homophilic and heterophilic cell-cell adhesion. CADM1 is expressed by a variety of cells including neurons, mast cells, spermatogonia, pancreatic islet cells, epithelial cells such as lung alveolar cells and biliary epithelial cells. CADM1 is involved in carcinoma pathogenesis and the expression of CADM1 is frequently down-regulated in various tumors derived from epithelial cells. It acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. (PMID: 18332875; 17326163; 20340131)