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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CAD Polyclonal antibody (16617-1-AP)

    WB analysis of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP (CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CAD Polyclonal antibody (16617-1-AP)

    IP experiment of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    IP result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CAD Polyclonal antibody (16617-1-AP)

    IHC staining of mouse liver using 16617-1-AP (same clone as 16617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CAD Polyclonal antibody (16617-1-AP)

    IHC staining of human liver cancer using 16617-1-AP (same clone as 16617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CAD Polyclonal antibody (16617-1-AP)

    IF Staining of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CAD Polyclonal antibody, PBS Only

CAD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16617-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16617-1-PBS targets CAD in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9912

Product name: Recombinant human CAD protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 502-851 aa of BC014178

Sequence: VAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

相同性解析による交差性が予測される生物種
Full Name carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Calculated molecular weight 2225 aa, 243 kDa
Observed molecular weight 240 kDa
GenBank accession numberBC014178
Gene Symbol CAD
Gene ID (NCBI) 790
RRIDAB_2878289
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP27708
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).

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