Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32066-1-AP targets CACTIN in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35645 Product name: Recombinant human CACTIN protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-188 aa of NM_021231 Sequence: MGRDTRSRSRSAGRRGRRRQSQSGSRSRSRSHGRRNRRRREDEGRRRRRRRSRERRSDSEEERWQRSGMRSRSPPRPKWHSRDGSSQSDSGEEQSRGQWARRRRRARSWSPSSSASSSASPGRSQSPRAAAAALSQQQSLQERLRLREERKQQEELMKAFETPEEKRARRLAKKEAKERKKREKMGWG 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 19 open reading frame 29 |
| Calculated molecular weight | 108 kDa, 89 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | NM_021231 |
| Gene Symbol | C19orf29 |
| Gene ID (NCBI) | 58509 |
| RRID | AB_3670183 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WUQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CACTIN plays a role in pre-mRNA splicing by facilitating excision of a subset of introns (PubMed:28062851).CACTIN was reported to be involved in the regulation of innate immune response (PubMed:20829348). CACTIN acts as a negative regulator of Toll-like receptor, interferon-regulatory factor (IRF) and canonical NF-kappa-B signaling pathways (PubMed:20829348, PubMed:26363554). CACTIN contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous pro-inflammatory stimuli (PubMed:20829348, PubMed:26363554). CACTIN has two isforms (89kDa and 108 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CACTIN antibody 32066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |