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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CACNB3 Recombinant antibody (84762-4-RR)

    IF Staining of MCF-7 using 84762-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CACNB3 antibody (84762-4-RR, Clone: 242088E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of SKOV-3 cells using CACNB3 Recombinant antibody (84762-4-RR)

    FC experiment of SKOV-3 using 84762-4-RR

    1x10^6 SKOV-3 cells were intracellularly stained with 0.25 ug CACNB3 Recombinant antibody (84762-4-RR, Clone:242088E4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84762-4-RR

    Affinity and Kinetic Characterization of 84762-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84762-4-RR against Human CACNB3 were performed. The affinity constant is 0.191 nM.

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CACNB3 Recombinant antibody

CACNB3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84762-4-RR
Clone No.242088E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

CACNLB3, CACNB 3, CAB3, 242088E4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84762-4-RR targets CACNB3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CACNB3 fusion protein Ag35651 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, beta 3 subunit
Calculated molecular weight55kDa，484aa
GenBank accession numberNM_000725.3
Gene Symbol CACNB3
Gene ID (NCBI) 784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP54284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACNB3, also known as CACNLB3, is a voltage-gated Ca2+ channel subunit. It has 5 isoforms and may play a role in the regulation of transcription factors and calcium transport.

Protocols

Product Specific Protocols
IF protocol for CACNB3 antibody 84762-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols