Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of mouse brain using 27453-1-AP
mouse brain tissue and mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 27453-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 27453-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 27453-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human brain using 27453-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 27453-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 27453-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 27453-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse eye using 27453-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CACNA2D1 Polyclonal antibody

CACNA2D1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 27453-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

CACNL2A, CCHL2A, MHS3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse skeletal muscle tissue, mouse cerebellum tissue, rat brain tissue, pig brain tissue
Positive IHC detected in	human skeletal muscle tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 2 publications below
IF	See 4 publications below
CoIP	See 1 publications below

Product Information

27453-1-AP targets CACNA2D1 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26190 Product name: Recombinant human CACNA2D1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-124 aa of NM_000722 Sequence: MEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASNEVVY 相同性解析による交差性が予測される生物種
Full Name	calcium channel, voltage-dependent, alpha 2/delta subunit 1
Calculated molecular weight	125 kDa
Observed molecular weight	125 kDa
GenBank accession number	NM_000722
Gene Symbol	CACNA2D1
Gene ID (NCBI)	781
RRID	AB_2880874
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54289
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CACNA2D1 antibody 27453-1-AP	Download protocol
IHC protocol for CACNA2D1 antibody 27453-1-AP	Download protocol
WB protocol for CACNA2D1 antibody 27453-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB,IF	Brain Behav Immun Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain. Authors - Khalil Ali Ahmad View Article
rat	WB,IF	Heliyon Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1 Authors - Chuan Jiang View Article
mouse	WB,IHC	iScience Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice Authors - Shan Gao View Article
human	WB,IHC	J Gastroenterol CACNA2D1 regulates the progression and influences the microenvironment of colon cancer Authors - Hiroyuki Inoue KD Validated View Article
mouse	WB,CoIP	Neurochem Res Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression Authors - Yunyi Huang View Article
human	WB	J Proteome Res ChIP-seq and RNA-seq Reveal the Involvement of Histone Lactylation Modification in Gestational Diabetes Mellitus Authors - Xiaman Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 27453-1-AP

WB analysis using 27453-1-AP

IHC staining of human skeletal muscle using 27453-1-AP

IHC staining of human skeletal muscle using 27453-1-AP

IHC staining of human brain using 27453-1-AP

IHC staining of human brain using 27453-1-AP

IF Staining of mouse eye using 27453-1-AP

IF Staining of mouse eye using 27453-1-AP

IF Staining of mouse eye using 27453-1-AP

CACNA2D1 Polyclonal antibody

CACNA2D1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain.

Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1

Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice

CACNA2D1 regulates the progression and influences the microenvironment of colon cancer

Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression

ChIP-seq and RNA-seq Reveal the Involvement of Histone Lactylation Modification in Gestational Diabetes Mellitus