Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
22279-1-AP targets CACNA1S in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | calcium channel, voltage-dependent, L type, alpha 1S subunit |
| Calculated molecular weight | 212 kDa |
| Observed molecular weight | 200-220 kDa |
| GenBank accession number | NM_000069 |
| Gene Symbol | CACNA1S |
| Gene ID (NCBI) | 779 |
| RRID | AB_2879058 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13698 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CACNA1S, also named as CACH1, CACN1 and CACNL1A3, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family and CACNA1S subfamily. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. The antibody is specific to CACNA1S.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CACNA1S antibody 22279-1-AP | Download protocol |
| WB protocol for CACNA1S antibody 22279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Reproduction Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion. | ||
bioRxiv Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM |
