Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28092-1-AP targets CACNA1H in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CACNA1H fusion protein Ag26094 相同性解析による交差性が予測される生物種 |
| Full Name | calcium channel, voltage-dependent, T type, alpha 1H subunit |
| Calculated molecular weight | 259 kDa |
| Observed molecular weight | 259 kDa |
| GenBank accession number | NM_001005407 |
| Gene Symbol | CACNA1H |
| Gene ID (NCBI) | 8912 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95180 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Voltage-dependent T-type calcium channel subunit alpha-1H (CACNA1H) is located in the plasma membrane of various tissues, including the brain cortex, amygdala, caudate nucleus, putamen, kidney, liver, and heart. This subunit regulates muscle contraction, secretion, neurotransmission, and gene expression by forming the pore (PMID: 33985586). Predominantly, the changes observed are missense mutations associated with gain-of-function, resulting in an increased influx of calcium into adrenal cells (PMID: 33985586; 37311830).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CACNA1H antibody 28092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |