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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACNA1E Polyclonal antibody (27225-1-AP)

    WB analysis using 27225-1-AP (same clone as 27225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27225-1-AP (CACNA1E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1E Polyclonal antibody (27225-1-AP)

    IHC staining of human brain using 27225-1-AP (same clone as 27225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1E Polyclonal antibody (27225-1-AP)

    IHC staining of human brain using 27225-1-AP (same clone as 27225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27225-1-PBS in a different storage buffer formulation.

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CACNA1E Polyclonal antibody, PBS Only

CACNA1E Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27225-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CACH6, CACNL1A6, Cav2.3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27225-1-PBS targets CACNA1E in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA1E fusion protein Ag25944 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, R type, alpha 1E subunit
Calculated molecular weight 262 kDa
Observed molecular weight240 kDa
GenBank accession numberNM_000721
Gene Symbol CACNA1E
Gene ID (NCBI) 777
RRIDAB_2880808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15878
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CACNA1E is a gene encoding the ion-conducting α1 subunit of R-type voltage-dependent calcium channels, genetic variability of CACNA1E is associated to risk of type 2 diabetes, insulin resistance and impaired insulin secretion in nondiabetic subjects.